Human BTNL8 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9359-100
MAB9359-SP
Human BTNL8 Antibody in Data
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Human BTNL8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTNL8 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2187B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell line HEK-293-derived Human BTNL8
Gln18-Lys238
Accession # Q6UX41-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
50 µg/mL
See below
Flow Cytometry
0.25 µg/5 x 106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human BTNL8 by Western Blot. View Larger

Detection of Human BTNL8 by Western Blot. Western blot shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with human BTNL8 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTNL8 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of BTNL8 in HEK293 Human Cell Line Transfected with Human BTNL8 and eGFP by Flow Cytometry. View Larger

Detection of BTNL8 in HEK293 Human Cell Line Transfected with Human BTNL8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human BTNL8 and eGFP was stained with either (A) Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Simple Western Detection of Human BTNL8 by Simple Western<sup>TM</sup>. View Larger

Detection of Human BTNL8 by Simple WesternTM. Simple Western lane view shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with with human BTNL8 and eGFP, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 66 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human BTNL8 by Simple Western<sup>TM</sup>. View Larger

Detection of Human BTNL8 by Simple WesternTM. Simple Western lane view shows lysates of human small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 60 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of
Ag-specific IgG during the primary but not the secondary immune response (3).

References
  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
  4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.
Long Name
Butyrophilin-like 8
Entrez Gene IDs
79908 (Human); 406160 (Rat)
Alternate Names
BTN9.2; BTNL8; butyrophilin-like 8; butyrophilin-like protein 8; UNQ702/PRO1347

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