Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2187B
View all BTNL8 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK-293-derived Human BTNL8
Gln18-Lys238
Accession # Q6UX41-1

Specificity

Detects human BTNL8 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human BTNL8 Antibody

Detection of Human BTNL8 antibody by Western Blot.

Detection of Human BTNL8 by Western Blot.

Western blot shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with human BTNL8 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTNL8 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of BTNL8 antibody in HEK293 Human Cell Line Transfected with Human BTNL8 and eGFP antibody by Flow Cytometry.

Detection of BTNL8 in HEK293 Human Cell Line Transfected with Human BTNL8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human BTNL8 and eGFP was stained with either (A) Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Detection of Human BTNL8 antibody by Simple WesternTM.

Detection of Human BTNL8 by Simple WesternTM.

Simple Western lane view shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with with human BTNL8 and eGFP, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 66 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human BTNL8 antibody by Simple WesternTM.

Detection of Human BTNL8 by Simple WesternTM.

Simple Western lane view shows lysates of human small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 60 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human BTNL8 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/5 x 106 cells
Sample: HEK293 Human Cell Line Transfected with Human BTNL8 and eGFP

Simple Western

50 µg/mL
Sample: Human small intestine tissue and Hek293T human embryonic kidney cell line either mock transfected or transfected with with human BTNL8 and eGFP

Western Blot

2 µg/mL
Sample: Hek293T human embryonic kidney cell line transfected with human BTNL8 and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of
Ag-specific IgG during the primary but not the secondary immune response (3).

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
  4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.

Long Name

Butyrophilin-like 8

Alternate Names

BTN9.2

Entrez Gene IDs

79908 (Human); 406160 (Rat)

Gene Symbol

BTNL8

UniProt

Q6UX41-1

Additional BTNL8 Products

Product Documents for Human BTNL8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BTNL8 Antibody

For research use only

Citations for Human BTNL8 Antibody

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