Human Bub1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Bub1 by Western Blot.
1 Image
Product Details
Supplemental Products

Human Bub1 Antibody Summary

Species Reactivity
Detects human Bub1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Bub1
Accession # O43683
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Bub1 antibody by Western Blot. View Larger

Detection of Human Bub1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and nuclear extracts of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Bub1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Bub1 was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bub1

The Bub1 (Budding uninhibited by benzimidazoles-1) protein is a kinase that acts in the mitotic spindle checkpoint. Bub1 delays chromosome segregation in response to genotoxic stress. Mutations or the absence of Bub1 can lead to aneuploidy and chromosomal instability.

Long Name
BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog
Entrez Gene IDs
699 (Human); 12235 (Mouse)
Alternate Names
BUB1 budding uninhibited by benzimidazoles 1 homolog; Bub1; BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1; BUB1L; budding uninhibited by benzimidazoles 1 (yeast homolog); budding uninhibited by benzimidazoles 1 homolog (yeast); EC; hBUB1mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Bub1 Antibody

There are currently no reviews for this product. Be the first to review Human Bub1 Antibody and earn rewards!

Have you used Human Bub1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review