Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all C1D Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human C1D
Ala2-Ser141
Accession # Q13901

Specificity

Detects human C1D in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human C1D Antibody

Detection of Human C1D antibody by Western Blot.

Detection of Human C1D by Western Blot.

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human C1D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4816) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for C1D at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human C1D Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: JEG‑3 human epithelial choriocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C1D

C1D (also SUN-CoR) is a 16 kDa member of the C1D family of DNA-binding proteins. It is ubiquitously expressed, activates DNA‑dependent protein kinase, and forms part of a large complex that processes rRNA. C1D exists as both a monomer and noncovalent homodimer, and is known to be phosphorylated. Human C1D is 141 amino acids (aa) in length. It contains two protein interaction sites at aa 50‑100 and 101‑141. There is one potential alternate site at Met54, and a number of single aa polymorphisms. Full-length C1D is 90% and 87% aa identical to mouse and canine C1D, respectively.

Long Name

C1D Nuclear DNA-binding Protein

Alternate Names

SUNCOR

Entrez Gene IDs

10438 (Human); 57316 (Mouse)

Gene Symbol

C1D

UniProt

Q13901

Additional C1D Products

Product Documents for Human C1D Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human C1D Antibody

For research use only

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