Detection of Human C1D by Western Blot. |
Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human C1D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4816) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for C1D at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
C1D (also SUN-CoR) is a 16 kDa member of the C1D family of DNA-binding proteins. It is ubiquitously expressed, activates DNA‑dependent protein kinase, and forms part of a large complex that processes rRNA. C1D exists as both a monomer and noncovalent homodimer, and is known to be phosphorylated. Human C1D is 141 amino acids (aa) in length. It contains two protein interaction sites at aa 50‑100 and 101‑141. There is one potential alternate site at Met54, and a number of single aa polymorphisms. Full-length C1D is 90% and 87% aa identical to mouse and canine C1D, respectively.
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