Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1031516
View all CA19-9 Primary Antibodies »

Product Specifications

Immunogen

Human CA19-9 from Capan-2 human pancreatic adenocarcinoma cell line

Specificity

Detects Human CA19-9 in ELISAs. 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CA19-9 Antibody

Detection of CA19-9 in Capan-2 Human Cell Line by Flow Cytometry.

Capan-2 human pancreatic adenocarcinoma cell line was stained with Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

CA19-9 in Capan‑2 Human Cell Line.

CA19-9 was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line using Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CA19-9 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Capan-2 human pancreatic adenocarcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CA19-9

CA19-9 (sialylated Lewis a antigen) is a serum tumor marker for pancreatic cancer diagnosis.  It is also used for monitoring therapy in patients with pancreatic adenocarcinoma.  CA19-9 is synthesized by normal pancreatic and biliary ductal cells and by gastric, colon, endometrial and saliva epithelia.  In a normal patient, it is present in small amounts in serum.  The plasmatic levels of CA19-9 are greatly increased in neoplastic disease.

Long Name

Carbohydrate Antigen 19-9

Alternate Names

CA 19-9

Additional CA19-9 Products

Product Documents for Human CA19-9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CA19-9 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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Protocols

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