Human CA19-9 Antibody

Catalog #: MAB10625 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10625-100
MAB10625-SP

Request a Quote Bulk Orders

Detection of CA19-9 in Capan-2 Human Cell Line by Flow Cytometry.

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CA19-9 Antibody Summary

Species Reactivity

Human

Specificity

Detects Human CA19-9 in ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1031516

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CA19-9 from Capan-2 human pancreatic adenocarcinoma cell line

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Capan-2 human pancreatic adenocarcinoma cell line

Immunocytochemistry

8-25 µg/mL

Immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CA19-9 in Capan-2 Human Cell Line by Flow Cytometry. Capan-2 human pancreatic adenocarcinoma cell line was stained with Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry

View Larger

CA19-9 in Capan‑2 Human Cell Line. CA19-9 was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line using Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CA19-9

CA19-9 (sialylated Lewis a antigen) is a serum tumor marker for pancreatic cancer diagnosis. It is also used for monitoring therapy in patients with pancreatic adenocarcinoma. CA19-9 is synthesized by normal pancreatic and biliary ductal cells and by gastric, colon, endometrial and saliva epithelia. In a normal patient, it is present in small amounts in serum. The plasmatic levels of CA19-9 are greatly increased in neoplastic disease.

Long Name

Carbohydrate Antigen 19-9

Alternate Names

CA 19-9; CA19-9