Human Calponin 1 Antibody

Catalog # Availability Size / Price Qty
AF7900
AF7900-SP
Calponin 1 in MDA‑MB‑231 Human Cell Line.
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Human Calponin 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calponin 1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human CNN-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Calponin 1
Ser2-Ala297
Accession # P51911
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Calponin 1 in MDA‑MB‑231 Human Cell Line. View Larger

Calponin 1 in MDA‑MB‑231 Human Cell Line. Calponin 1 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Sheep Anti-Human Calponin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7900) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Calponin 1 in Human Small Intestine. View Larger

Calponin 1 in Human Small Intestine. Calponin 1 was detected in immersion fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human Calponin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7900) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calponin 1

CNN-1 (calponin-1 [calcium and calmodulin-binding troponin T-like protein]; also calponin basic, CaP and calponin H1) is a 32-36 kDa cytoplasmic member of the calponin family of proteins. Although reportedly expressed in fibroblasts and endothelial cells, it actually appears to be restricted to smooth muscle and smooth muscle-like cells such as myoepithalium and myofibroblasts in the adult. CNN-1 interacts with F-actin in a phosphorylation-dependent manner. When nonphosphorylated, CNN-1 blocks actomyosin ATPase activity, contributing to the stabilization of actin stress fiber bundles. Thus, CNN-1 expression inhibits cell motility and the formation of podosomes. Human CNN-1 is 297 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 30-127), and three consecutive calponin-like repeats (aa 164-268). The repeats are suggested to mediate actin binding. There are five potential Ser/Thr phosphorylation sites. Full-length human CNN-1 shares 97% aa sequence identity with mouse CNN-1.

Entrez Gene IDs
1264 (Human); 12797 (Mouse); 65204 (Rat)
Alternate Names
Basic calponin; Calponin 1; calponin 1, basic, smooth muscle; Calponin H1; Calponin H1, smooth muscle; calponins, basic; CNN1; Sm-Calp; SMCC; SMCCcalponin-1

Product Datasheets

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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