Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 681207
View all Calreticulin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Calreticulin
Glu18-Leu417
Accession # P27797

Specificity

Detects human Calreticulin in direct ELISAs. In direct ELISAs, no cross-reactivity with human Vasostatin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Calreticulin Antibody

Detection of Human Calreticulin antibody by Western Blot.

Detection of Human Calreticulin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Calreticulin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Calreticulin antibody in Human Prostate by Immunohistochemistry (IHC-P).

Calreticulin in Human Prostate.

Calreticulin was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38982) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Calreticulin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MOLT‑4 human acute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calreticulin

Human Calreticulin is a 55‑60 kDa, 400 amino acid, variably glycosylated intra- and extracellular Ca++-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.

Alternate Names

Autoantigen Ro, CALR, Calregulin, cC1qR, CRT, SSA, Vasostatin

Entrez Gene IDs

811 (Human); 12317 (Mouse); 64202 (Rat)

Gene Symbol

CALR

UniProt

P27797 (Human)

Additional Calreticulin Products

Product Documents for Human Calreticulin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Calreticulin Antibody

For research use only

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Protocols

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