Human Carbohydrate Sulfotransferase 5/CHST5 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Carbohydrate Sulfotransferase 5/CHST5 by Western blot.
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Product Details
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Human Carbohydrate Sulfotransferase 5/CHST5 Antibody Summary

Species Reactivity
Detects human Carbohydrate Sulfotransferase 5/CHST5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST2, rmCHST5, and rmCHST7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 5/CHST5
Accession # Q9GZS9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 5/CHST5, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Carbohydrate Sulfotransferase 5/CHST5 antibody by Western Blot. View Larger

Detection of Human Carbohydrate Sulfotransferase 5/CHST5 by Western blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 5/CHST5

CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha ) is likely a 53‑56 kDa member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position # 6 of mucin-associated GlcNAc. Human CHST5 is a 390 amino acid (aa) type II transmembrane protein. It contains a short cytoplasmic segment (aa 1‑9) and a long luminal domain (aa 28‑390) that contains an enzymatic region (aa 180‑358). There is one alternate start site that is 22 aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa 28‑390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4-beta. Over aa 28‑390, human GST4‑ alpha /CHST5 is 78% aa identical to mouse GST4.

Entrez Gene IDs
23563 (Human); 56773 (Mouse); 307859 (Rat)
Alternate Names
carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5; Carbohydrate Sulfotransferase 5; CHST5; GlcNAc6ST3; gn6st-3; GST4-alpha; hIGn6ST; I-GlcNAc6ST

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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