Human Carbohydrate Sulfotransferase 5/CHST5 Antibody

Catalog #: AF5446 Datasheet / COA / SDS

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AF5446
AF5446-SP

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Detection of Human Carbohydrate Sulfotransferase 5/CHST5 by Western blot.

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Human Carbohydrate Sulfotransferase 5/CHST5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbohydrate Sulfotransferase 5/CHST5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST2, rmCHST5, and rmCHST7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 5/CHST5
Ser28-Arg390
Accession # Q9GZS9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 5/CHST5, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Carbohydrate Sulfotransferase 5/CHST5 by Western blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 5/CHST5

CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha ) is likely a 53‑56 kDa member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position # 6 of mucin-associated GlcNAc. Human CHST5 is a 390 amino acid (aa) type II transmembrane protein. It contains a short cytoplasmic segment (aa 1‑9) and a long luminal domain (aa 28‑390) that contains an enzymatic region (aa 180‑358). There is one alternate start site that is 22 aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa 28‑390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4-beta. Over aa 28‑390, human GST4‑ alpha /CHST5 is 78% aa identical to mouse GST4.

Entrez Gene IDs

23563 (Human); 56773 (Mouse); 307859 (Rat)

Alternate Names

carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5; Carbohydrate Sulfotransferase 5; CHST5; GlcNAc6ST3; gn6st-3; GST4-alpha; hIGn6ST; I-GlcNAc6ST