Human Carbonic Anhydrase II/CA2 Antibody Summary
Accession # P00918
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Carbonic Anhydrase II/CA2 by Western Blot. Western blot shows lysates of human heart tissue, human kidney tissue, Caki-2 human clear cell carcinoma epithelial cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/ CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbonic Anhydrase II/ CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase II/CA2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and Caco-2 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 37 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase II/CA2
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2).
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Shah, G.N. et al. (2004) Hum. Mutat. 24:272.
Citation for Human Carbonic Anhydrase II/CA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing
Authors: P Wang, Y Chen, J Yong, Y Cui, R Wang, L Wen, J Qiao, F Tang
Cell Rep, 2018;24(13):3554-3567.e3.
Sample Types: Whole Tissue
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