Human Carbonic Anhydrase II/CA2 Antibody

R&D Systems | Catalog # AF2184

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Carbonic Anhydrase II/CA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase II/CA2
Ser2-Lys260
Accession # P00918

Specificity

Detects human Carbonic Anhydrase II/CA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase I, III, and XIII is observed and less than 1% cross-reactivity with rhCarbonic Anhydrase IV, VIII, IX, X, XII, and XIV is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Carbonic Anhydrase II/CA2 Antibody

Detection of Human Carbonic Anhydrase II/CA2 antibody by Western Blot.

Detection of Human Carbonic Anhydrase II/CA2 by Western Blot.

Western blot shows lysates of human heart tissue, human kidney tissue, Caki-2 human clear cell carcinoma epithelial cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/ CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbonic Anhydrase II/ CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase II/CA2 antibody by Simple WesternTM.

Detection of Human Carbonic Anhydrase II/CA2 by Simple WesternTM.

Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and Caco-2 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 37 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Carbonic Anhydrase II/CA2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum) and spinal cord

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase II (Catalog # 2184-CA), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: Human kidney tissue, human heart tissue, and Caco‑2 human colorectal adenocarcinoma cell line

Western Blot

1 µg/mL
Sample: Human heart tissue, human kidney tissue, Caki‑2 human clear cell carcinoma epithelial cell line, and A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Carbonic Anhydrase II/CA2

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Shah, G.N. et al. (2004) Hum. Mutat. 24:272.

Alternate Names

CA2

Entrez Gene IDs

760 (Human)

Gene Symbol

CA2

UniProt

P00918

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Human Carbonic Anhydrase II/CA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Carbonic Anhydrase II/CA2 Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Human Carbonic Anhydrase II/CA2 Antibody

Customer Reviews for Human Carbonic Anhydrase II/CA2 Antibody

There are currently no reviews for this product. Be the first to review Human Carbonic Anhydrase II/CA2 Antibody and earn rewards!

Have you used Human Carbonic Anhydrase II/CA2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies