Human Carbonic Anhydrase II/CA2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Carbonic Anhydrase II/CA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase I, III, and XIII is observed and less than 1% cross-reactivity with rhCarbonic Anhydrase IV, VIII, IX, X, XII, and XIV is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Carbonic Anhydrase II/CA2
    Ser2-Lys260
    Accession # P00918
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human brain (cerebellum) and spinal cord
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase II (Catalog # 2184-CA), see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Carbonic Anhydrase II/CA2 by Western Blot. Western blot shows lysates of human heart tissue, human kidney tissue, Caki‑2 human clear cell carcinoma epithelial cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/
CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbonic Anhydrase II/
CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase II/CA2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and Caco‑2 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 37 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase II/CA2

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2).

  • References:
    1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
    2. Shah, G.N. et al. (2004) Hum. Mutat. 24:272.
  • Entrez Gene IDs:
    760 (Human)
  • Alternate Names:
    CA2; CAII; CA-IIEC 4.2.1.1; Car2; Carbonate dehydratase II; carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; Carbonic Anhydrase II; carbonic anhydrase IICAC; carbonic dehydratase
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