|Detection of Human Carbonic Anhydrase VI by Western Blot. Western blot shows human saliva. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of Human Carbonic Anhydrase VI by Simple WesternTM. Simple Western lane view shows human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.|
Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5). Mature human CA6 shares 66% and 50% amino acid sequence identity with bovine and mouse CA6, respectively.
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