Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 401819
View all Carbonic Anhydrase VI Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606

Specificity

Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Carbonic Anhydrase 2, 3, 4, 5A, 5B, 7, 8, 9, recombinant mouse Carbonic Anhydrase 12, and 14 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Carbonic Anhydrase VI Antibody

Detection of Human Carbonic Anhydrase VI antibody by Western Blot.

Detection of Human Carbonic Anhydrase VI by Western Blot.

Western blot shows human saliva. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase VI antibody by Simple WesternTM.

Detection of Human Carbonic Anhydrase VI by Simple WesternTM.

Simple Western lane view shows human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Carbonic Anhydrase VI Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: Human saliva

Western Blot

1 µg/mL
Sample: Human saliva

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase VI

Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5). Mature human CA6 shares 66% and 50% amino acid sequence identity with bovine and mouse CA6, respectively.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
  3. Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
  4. Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
  5. Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.

Alternate Names

CA6, Gustin

Entrez Gene IDs

765 (Human); 298657 (Rat)

Gene Symbol

CA6

UniProt

EAW71606

Additional Carbonic Anhydrase VI Products

Product Documents for Human Carbonic Anhydrase VI Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carbonic Anhydrase VI Antibody

For research use only

Citations for Human Carbonic Anhydrase VI Antibody

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