Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carboxypeptidase A1/CPA1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A1/CPA1
Lys17-Tyr419
Accession # P15085

Specificity

Detects human Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant mouse CPA1 is observed and less than 10% cross-reactivity with recombinant human CPB1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Carboxypeptidase A1/CPA1 Antibody

Neutralization by Human CPA1 Antibody

Measured by its ability to neutralize Recombinant Human Carboxypeptidase A1/CPA1 (0.1 µg/mL, 2856-ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM). The Neutralization Dose (ND50) is

Applications for Human Carboxypeptidase A1/CPA1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN)

Neutralization

Measured by its ability to neutralize Recombinant Human Carboxypeptidase A1/CPA1 (0.1 µg/mL, Catalog # 2856-ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM).  The Neutralization Dose (ND50) is <15 µg/mL. 

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Carboxypeptidase A1/CPA1

Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified rhCPA1 corresponds to the pro form which can be activated.

References

  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 812, Academic Press, San Diego.

Alternate Names

CPA1

Entrez Gene IDs

1357 (Human); 109697 (Mouse)

Gene Symbol

CPA1

UniProt

P15085

Additional Carboxypeptidase A1/CPA1 Products

Product Documents for Human Carboxypeptidase A1/CPA1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Carboxypeptidase A1/CPA1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Human Carboxypeptidase A1/CPA1 Antibody

There are currently no reviews for this product. Be the first to review Human Carboxypeptidase A1/CPA1 Antibody and earn rewards!

Have you used Human Carboxypeptidase A1/CPA1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies