Human Carboxypeptidase A2/CPA2 Antibody

Catalog # Availability Size / Price Qty
Product Details
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Human Carboxypeptidase A2/CPA2 Antibody Summary

Species Reactivity
Detects human Carboxypeptidase A2/CPA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA4, rhCPB1, or rhCPE is observed. Recognizes the pro form (aa 17‑417), but not the activated enzyme (aa 113‑417) in Western blots.
Monoclonal Mouse IgG2B Clone # 384023
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2
Accession # P48052
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Carboxypeptidase A2/CPA2 (Catalog # 2896-ZN)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A2/
CPA2 (Catalog # 2896-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase A2/CPA2

Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (aa 1 to 16), a pro region (aa 17 to 112), and a mature chain (aa 113 to 417). The amino acid sequence of human CPA2 is 99.5%, 90%, 87%, 86% and 70% identical to that of chimpanzee, canine, rat, mouse and chicken.

  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.
Entrez Gene IDs
1358 (Human)
Alternate Names
carboxypeptidase A2 (pancreatic); Carboxypeptidase A2; CPA2; EC 3.4.17; EC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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