Human Carboxypeptidase B2/CPB2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Carboxypeptidase A1, A4, recombinant mouse (rm) Carboxypeptidase A1, A4, B1, and 5% cross-reactivity with rhCarboxypeptidase B1, B2, and E is observed under reducing conditions. No cross-reactivity with any of these proteins is observed under nonreducing conditions.
  • Source
    Monoclonal Mouse IgG1 Clone # 650801
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B2/CPB2
    Phe23-Val423 (predicted)
    Accession # Q96IY4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human  Carboxypeptidase B2/CPB2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Carboxypeptidase B2/CPB2 Monoclonal Antibody (Catalog # MAB6036) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Carboxypeptidase B2/CPB2 at approximately 45 and 50 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxypeptidase B2/CPB2

CPB2 (Carboxypeptidase B2; also CPU and TAFI) is a secreted, 50-60 kDa glycoprotein member of the peptidase M14 family of enzymes.  It is expressed by hepatocytes (60 kDa) and platelets (50 kDa), with MW differences attributable to glycosylation.  CPB2 is cleaved by thrombin and plasmin, generating a 36 kDa, relatively insoluble nonglycosylated enzymatically active fragment (TAFIa).  Active CPB2 removes C-terminal Lys residues from fibrin, thereby interrupting plasmin generation and promoting fibrin polymerization.  Human CPB2 (proprecursor/zymogen) is 401 amino acids (aa) in length.  It contains a prosequence (aa 23-114) and an active fragment (aa 115-423) that acts on C-terminal Lys or Arg residues.  There is one potential isoform variant that shows a deletion of aa 198-234 accompanied by a 16 aa substitution for aa 382-423.  Over aa 23-423, human CPB2 shares 85% aa identity with mouse CPB2.  

  • Entrez Gene IDs:
    1361 (Human); 56373 (Mouse); 113936 (Rat)
  • Alternate Names:
    carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); Carboxypeptidase B2; Carboxypeptidase U; CPB2; CPU; CPUEC 3.4.17.20; EC 3.4.17; PCPB; Plasma carboxypeptidase B; TAFI; TAFIcarboxypeptidase B-like protein; Thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
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