Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 420709
View all Carboxypeptidase E/CPE Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase E/CPE
Arg42-Ser453
Accession # P16870

Specificity

Detects human Carboxypeptidase E/CPE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA2, rhCPB1, or recombinant mouse CPA4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Carboxypeptidase E/CPE Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxypeptidase E/CPE

Carboxypeptidase E (CPE) is a peripheral membrane protein that cleaves C-terminal amino acid residues and is involved in the processing of numerous peptide hormones and neurotransmitters. CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance. The CPE precursor includes a signal peptide (aa 1‑25), a propeptide (aa 26‑42) and a mature chain (aa 43‑476). The amino acid sequence of human CPE is 99.8%, 96.6%, 96.3%, and 90.2% identical to that of chimpanzee, mouse, dog and chicken.

Alternate Names

CPE, CPH-1

Entrez Gene IDs

1363 (Human); 12876 (Mouse); 25669 (Rat)

Gene Symbol

CPE

UniProt

P16870

Additional Carboxypeptidase E/CPE Products

Product Documents for Human Carboxypeptidase E/CPE Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Carboxypeptidase E/CPE Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators
Obesity

Citations for Human Carboxypeptidase E/CPE Antibody

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