Human Carboxypeptidase M Antibody

Catalog # Availability Size / Price Qty
AF7457
AF7457-SP
Detection of Human Carboxypeptidase M by Western Blot.
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Human Carboxypeptidase M Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carboxypeptidase M in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CPE is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Carboxypeptidase M
Leu18-Ser423
Accession # P14384
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Carboxypeptidase M by Western Blot. View Larger

Detection of Human Carboxypeptidase M by Western Blot. Western blot shows lysates of human placenta tissue and human adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carboxypeptidase M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxypeptidase M at approximately 58-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Carboxypeptidase  M in THP‑1 Human Cell Line. View Larger

Carboxypeptidase M in THP‑1 Human Cell Line. Carboxypeptidase M was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Sheep Anti-Human Carboxypeptidase M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7457) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Carboxypeptidase M in Human Placenta. View Larger

Carboxypeptidase M in Human Placenta. Carboxypeptidase M was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Carboxypeptidase M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7457) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane of decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase M

CPM (Carboxypeptidase M) is a 50‑65 kDa monomer that belongs to the regulatory CPN/E subfamily, peptidase M14 family of enzymes. It is widely expressed, being found on macrophages, fibroblasts, endothelial cells, oligodendrocytes and Schwann cells, dendritic cells, osteoblasts and bronchial epithelium. CPM is a GPI‑linked glycoprotein that is best known as a peptidase that cleaves basic amino acids (aa) from the carboxyterminal of a number of peptides, including EGF and bradykinin. It is also known to bind to apparent substrates and undergo a conformational change that links it with the kinin B1 GPCR, initiating signal transduction. Mature human CPM is 406 aa in length (aa 18-423). It contains one large enzymatic region (aa 19-310) and two critical glutamic acid residues at Glu260 and Glu264. Like many GPI‑linked proteins, CPM undergoes solubilization and is reportedly found in urine and amniotic fluid. Over aa 18-423 (mature CPM), human CPM shares 86% aa sequence identity with mouse CPM.

Entrez Gene IDs
1368 (Human); 70574 (Mouse); 314855 (Rat)
Alternate Names
Carboxypeptidase M; CPM; EC 3.4.17; EC 3.4.17.12

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Immunohistochemistry Reagents

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Secondary Antibodies

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