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Human Cardiotrophin-1/CT-1 Antibody

Catalog #: MAB2601
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2601-SP
MAB2601-500
MAB2601-100

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Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody.
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Human Cardiotrophin-1/CT-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 89215
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human Cardiotrophin‑1/CT‑1
Ser2-Ala201
Accession # Q16619
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD)
Neutralization
Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 20 ng/mL Recombinant Human Cardiotrophin‑1/CT‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody. View Larger

Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody. Recombinant Human Cardiotrophin-1/CT-1 (Catalog # 612-CD) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Cardiotrophin-1/CT-1 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Cardiotrophin-1/CT-1 Mono- clonal Antibody (Catalog # MAB2601). The ND50 is typically 1-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third  alpha receptor subunit has been implicated in the receptor complex.

References
  1. Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
  2. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.
Entrez Gene IDs
1489 (Human); 13019 (Mouse)
Alternate Names
cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1

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Citation for Human Cardiotrophin-1/CT-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
    Authors: Gard AL, Gavin E, Solodushko V, Pennica D
    Neuroscience, 2004-01-01;127(1):43-52.
    Species: Mouse, Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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