Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 674144
View all CCR11 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CCR11
Accession # Q9NPB9

Specificity

Detects human CCR11 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human CCR11 Antibody

Detection of CCR11 antibody in HEK293 Human Cell Line Transfected with Human CCR11 and eGFP antibody by Flow Cytometry.

Detection of CCR11 in HEK293 Human Cell Line Transfected with Human CCR11 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human CCR11 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CCR11 Monoclonal Antibody (Catalog # MAB6899) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.

Applications for Human CCR11 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human CCR11 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR11

CCR11, also known as CCRL1 and CCX-CKR, is a 45 kDa 7-transmembrane segment protein that is widely expressed on stromal cells. It is a silent receptor that does not induce intracellular calcium signaling, but instead serves as a chemokine scavenger receptor. CCR11 regulates dendritic cell and thymic precursor migration by mediating the clearance of the homeostatic chemokines CCL19, 21, and 25. It is upregulated on some breast cancer cells and on bronchial epithelial cells in pulmonary sarcoidosis. Human CCR11 shares 85% amino acid sequence identity with mouse and rat CCR11.

Alternate Names

ACKR4, CC-CKR-11, CCRL1, CCX-CKR, PPR1, VSHK1

Entrez Gene IDs

51554 (Human); 252837 (Mouse); 685243 (Rat)

Gene Symbol

ACKR4

UniProt

Q9NPB9

Additional CCR11 Products

Product Documents for Human CCR11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCR11 Antibody

For research use only

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