Human CD1e Antibody
Human CD1e Antibody Summary
Accession # P15812
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD1e in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was treated for 3 hours with monensin, then stained with Mouse Anti-Human CD1e Monoclonal Antibody (Catalog # MAB7330, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD1e is an approximately 40 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1e associates with beta 2-microglobulin in endosomes and the Golgi where it facilitates the processing of glycolipids for their presentation by other CD1 family proteins. CD1e is expressed by most nonhuman mammals but not by mice or rats. It contains one Ig-like domain in its extracellular region. Alternate splicing of human CD1e generates multiple isoforms with various deletions and substitutions in the extracellular and/or cytoplasmic domains.
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