Human CD1e Antibody

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Detection of CD1e in Jurkat Human Cell Line by Flow Cytometry.
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Human CD1e Antibody Summary

Species Reactivity
Detects human CD1e in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1d, or recombinant rat beta 2-Microglobulin is observed.
Monoclonal Mouse IgG1 Clone # 704407
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human CD1e
Accession # P15812
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CD1e antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD1e in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was treated for 3 hours with monensin, then stained with Mouse Anti-Human CD1e Monoclonal Antibody (Catalog # MAB7330, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD1e

CD1e is an approximately 40 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1e associates with beta 2-microglobulin in endosomes and the Golgi where it facilitates the processing of glycolipids for their presentation by other CD1 family proteins. CD1e is expressed by most nonhuman mammals but not by mice or rats. It contains one Ig-like domain in its extracellular region. Alternate splicing of human CD1e generates multiple isoforms with various deletions and substitutions in the extracellular and/or cytoplasmic domains.

Entrez Gene IDs
913 (Human)
Alternate Names
CD1-alpha-3; CD1e molecule; CD1e; CDIEL; e polypeptide; FLJ17609; membrane-associated; R2G1; thymocyte antigen CD1E

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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