|Detection of Human CD21 by Western Blot. Western blot shows lysates of human spleen tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CD21 Monoclonal Antibody (Catalog # MAB4909) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD21 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of CD21 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD21 Monoclonal Antibody (Catalog # MAB4909) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).|
CD21 (also called EBV receptor or CR2) is a 145 kDa member of the RCA (receptors of complement activation) family of proteins. It is expressed on T cells, B cells, and follicular dendritic cells. On the B cell surface, it combines with the BCR and CD19 to form a B cell activating complex. Mature human CD21 is 1013 amino acids (aa) in length. It is a type I transmembrane (TM) protein that contains a 951 aa extracellular domain (ECD) (aa 21-971) and a short 34 aa cytoplasmic tail. The ECD exhibits fifteen 60 aa SUSHI repeats. Soluble CD21 can be generated by cleavage near the TM domain. One potential splice variant shows a deletion of aa 847‑908, while another shows an insertion of 59 aa after Lys659. The ECD of human CD21 shares 71% aa identity with mouse CD21 ECD.
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