Human CD27/TNFRSF7 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human CD27 / TNFRSF7 ELISA Standard Curve
Preparation and Storage
Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is induced by antigen-receptor activation in B cells.
Citations for Human CD27/TNFRSF7 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration
Authors: K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López
PLoS ONE, 2020;15(8):e0236283.
Sample Types: Plasma
A Predictive 7-Gene Assay and Prognostic Protein Biomarkers for Non-small Cell Lung Cancer
Authors: NL Guo, A Dowlati, RA Raese, C Dong, G Chen, DG Beer, J Shaffer, S Singh, U Bokhary, L Liu, J Howington, T Hensing, Y Qian
Sample Types: Tissue Homogenates
Integration of magnetic resonance imaging and protein and metabolite CSF measurements to enable early diagnosis of secondary progressive multiple sclerosis
Authors: S Herman, PE Khoonsari, A Tolf, J Steinmetz, H Zetterberg, T Åkerfeldt, PJ Jakobsson, A Larsson, O Spjuth, J Burman, K Kultima
Sample Types: CSF
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