Human CD27/TNFRSF7 DuoSet ELISA

R&D Systems | Catalog # DY382-05

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

125-8000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human CD27/TNFRSF7 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all CD27/TNFRSF7 ELISA Kits »

Product Summary for Human CD27/TNFRSF7 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CD27 / TNFRSF7. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human CD27/TNFRSF7 DuoSet ELISA

Human CD27 / TNFRSF7 ELISA Standard Curve

Human CD27 / TNFRSF7 ELISA Standard Curve

Kit Contents for Human CD27/TNFRSF7 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is induced by antigen-receptor activation in B cells.

Alternate Names

CD27, TNFRSF7

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Gene Symbol

CD27

Additional CD27/TNFRSF7 Products

Product Documents for Human CD27/TNFRSF7 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD27/TNFRSF7 DuoSet ELISA

For research use only

Citations for Human CD27/TNFRSF7 DuoSet ELISA

Customer Reviews for Human CD27/TNFRSF7 DuoSet ELISA (1)

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  • Human CD27/TNFRSF7 DuoSet ELISA
    Name: Anonymous
    Sample Tested: human serum and plasma
    Verified Customer | Posted 04/02/2020
    We used this kit for the quantification of CD27 in human serum and plasma. Works very well and well-described protocol.
    Human CD27/TNFRSF7 DuoSet ELISA DY382-05

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