Ectonucleoside triphosphate diphosphohydrolase-6 (NTPDase-6) is a secreted nucleoside phosphohydrolase of the CD39 family of enzymes (1). hNTPDase6 displays a preference for the nucleoside-5’-diphosphates GDP and IDP over CDP and UDP (2). Nucleoside-5’-triphosphates are also hydrolyzed, but at much lower rates. hNTPDase6 has a broad tissue distribution, its mRNA has been detected in all human tissues tested (1).
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD39L2/ENTPD6
Lys61-Ser484
Accession # O75354
Lys61-Ser484
Accession # O75354
Specificity
Detects human CD39L2/ENTPD6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) CD39 and rhCD39L3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human CD39L2/ENTPD6 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human CD39L2/ENTPD6 (Catalog # 4399-EN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human CD39L2/ENTPD6 (Catalog # 4399-EN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human CD39L2/ENTPD6 (Catalog # 4399-EN)
Sample: Recombinant Human CD39L2/ENTPD6 (Catalog # 4399-EN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD39L2/ENTPD6
References
- Chadwick, B.P. and A.M. Frischauf (1998) Genomics. 50:357.
- Hicks-Berger, C.A. et al. (2000) J. Biol. Chem. 275:34041.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 6
Alternate Names
CD39L2, ENTPD6, NTPD3, NTPDase 6
Gene Symbol
ENTPD6
UniProt
Additional CD39L2/ENTPD6 Products
Product Documents for Human CD39L2/ENTPD6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CD39L2/ENTPD6 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars