Human CEACAM-16 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9885-100
MAB9885-SP
Detection of CEACAM-16 in HEK293 Human Cell Line Transfected with Human CEACAM-16 by Flow Cytometry
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Human CEACAM-16 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-16 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2747B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived human CEACAM-16
Met1-Gly425
Accession # Q2WEN9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line Transfected with Human CEACAM-16
Immunocytochemistry
3-25 µg/mL
Immersion fixed HEK293 human embryonic kidney cell line transfected

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry View Larger

Detection of CEACAM-16 in HEK293 Human Cell Line Transfected with Human CEACAM-16 by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human CEACAM-16 (filled histogram) or irrelevant protein (open histogram) was stained with Rabbit Anti-Human CEACAM-16 Monoclonal Antibody (Catalog # MAB9885) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry View Larger

CEACAM-16 in HEK293 Human Cell Line. CEACAM-16 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rabbit Anti-Human CEACAM-16 Monoclonal Antibody (Catalog # MAB9885) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-16

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16 (CEACAM-16), or CEAL2, is part of the CEA protein family consisting of CEACAMs and the pregnancy‑specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Unique to the CEA family, CEACAM-16 is a secreted molecule lacking a recognizable transmembrane domain or GPI anchor (3). Mature human CEACAM-16 is 405 amino acids (aa), containing 2 IgC2-like domains and 2 IgV-like domains. CEACAM-16 is one of only five conserved CEACAMs among mice, rats, and humans (2). Mature human CEACAM-16 shares 90% and 89% aa identity with mouse and rat CEACAM‑16, respectively. Originally discovered as a biomarker for colorectal cancer (4), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5-7). CEACAM-16 is specifically expressed in the inner ear and has been shown to play a critical role in hearing. CEACAM-16 has been identified as a binding partner for alpha tectorin and specific mutations in CEACAM-16 have been linked to autosomal dominant nonsyndromic deafness (ADNSHL) (3, 8).

References
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser R. et al. (2005) Genomics 86:566.
  3. Zheng, J. et al. (2011) PNAS. 108(10):4218.
  4. Gold P. and Freedman S.O. (1965) J Exp Med 122:467.
  5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  6. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  7. Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
  8. Wang, H. et al. (2015) J Hum Genet. 60(3):119.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 16
Entrez Gene IDs
388551 (Human); 330483 (Mouse); 292700 (Rat)
Alternate Names
carcinoembryonic antigen-related cell adhesion molecule 16; CEACAM-16; CEAL2; DFNA4B

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