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Human Chordin-like 2/CHRDL2 Antibody

MAB24481 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB24481
MAB24481-SP
Product Details
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Supplemental Products
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chordin-like 2/CHRDL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse CHRDL1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 272905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chordin-like 2/CHRDL2
Arg28-Thr451
Accession # NP_056239
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Chordin-like 2/CHRDL2
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Entrez Gene IDs
25884 (Human); 69121 (Mouse)
Alternate Names
BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2

Product Datasheets

Product Datasheet
COA
SDS

FAQs

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