CIN85/SH3KBP1 in A549 Human Cell Line. CIN85/SH3KBP1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CIN85/SH3KBP1 Monoclonal Antibody (Catalog # MAB88771) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Independently identified by several groups, SH3-domain kinase-binding protein 1 (SH3KBP1) is also known as CBL-interacting protein of 85 kDa (CIN85), CD2-binding protein 3 (CD2BP3) and human src family kinase-binding protein 1 (HSB1). SH3KBP1 is a widely expressed adaptor protein involved in regulating diverse signal transduction pathways, including endocytosis, lysosomal degradation, cell adhesion, stress response, cell morphology and cytoskeletal organization. The protein contains three N-terminal SH3 domains for complex assembly and a C-terminal coiled coil domain for oligomerization, separated by a proline-rich central region. Smaller known isoforms of SH3KBP1 lack one or more of the SH3 domains. Over amino acids (aa) 366-665, human SH3KBP1 shares 92% and 90% aa identity with mouse and rat SH3KBP1, respectively.
Cbl-Interacting Protein of 85 KDa
Entrez Gene IDs:
30011 (Human); 58194 (Mouse); 84357 (Rat)
C-Cbl-Interacting Protein; CD2-binding protein 3; CD2BP3; CIN85; CIN85Cbl-interacting protein of 85 kDa; GIG10; HSB1; HSB-1; Human Src family kinase-binding protein 1; MIG18; migration-inducing gene 18; SH3 domain-containing kinase-binding protein 1; SH3-domain kinase binding protein 1; SH3KBP1; Src family kinase-binding protein 1; src-related kinase binding protein-1
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.