Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 931346
View all CIN85/SH3KBP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CIN85/SH3KBP1
Pro366-Lys665
Accession # Q96B97

Specificity

Detects human CIN85/SH3KBP1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CIN85/SH3KBP1 Antibody

CIN85/SH3KBP1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

CIN85/SH3KBP1 in A549 Human Cell Line.

CIN85/SH3KBP1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CIN85/SH3KBP1 Monoclonal Antibody (Catalog # MAB88771) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CIN85/SH3KBP1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CIN85/SH3KBP1

Independently identified by several groups, SH3-domain kinase-binding protein 1 (SH3KBP1) is also known as CBL-interacting protein of 85 kDa (CIN85), CD2-binding protein 3 (CD2BP3) and human src family kinase-binding protein 1 (HSB1).  SH3KBP1 is a widely expressed adaptor protein involved in regulating diverse signal transduction pathways, including endocytosis, lysosomal degradation, cell adhesion, stress response, cell morphology and cytoskeletal organization.  The protein contains three N-terminal SH3 domains for complex assembly and a C-terminal coiled coil domain for oligomerization, separated by a proline-rich central region.  Smaller known isoforms of SH3KBP1 lack one or more of the SH3 domains.  Over amino acids (aa) 366-665, human SH3KBP1 shares 92% and 90% aa identity with mouse and rat SH3KBP1, respectively.

Long Name

Cbl-Interacting Protein of 85 KDa

Alternate Names

C-Cbl-Interacting Protein, CD2BP3, GIG10, HSB-1, MIG18, SH3KBP1

Entrez Gene IDs

30011 (Human); 58194 (Mouse); 84357 (Rat)

Gene Symbol

SH3KBP1

UniProt

Q96B97

Additional CIN85/SH3KBP1 Products

Product Documents for Human CIN85/SH3KBP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CIN85/SH3KBP1 Antibody

For research use only

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