Human CLC Antibody

Catalog # Availability Size / Price Qty
AF962
AF962-SP
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Human CLC Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CT-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CLC
Leu28-Phe225
Accession # Q9UBD9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CLC (Catalog # 962-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLC

Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8).  Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

References
  1. Murakami, M. et al. (2004) Growth Factors 22:75.
  2. Ernst, M. & B.J. Jenkins (2004) Trends Genet. 20:23.
  3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
  4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
  5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
  6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
  7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
  8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
  9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854.
  10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
  11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
  12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.
Long Name
Cardiotrophin-like Cytokine
Entrez Gene IDs
23529 (Human); 56708 (Mouse); 365395 (Rat)
Alternate Names
B-cell stimulating factor 3; B-cell stimulatory factor 3; BSF-3; BSF3BSF-3; cardiotrophin-like cytokine factor 1; CISS2; CLC; CLCF1; CLCNNT-1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT-1; NNT1B-cell-stimulating factor 3; Novel neurotrophin-1; NR6

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