Human Coagulation Factor X Antibody

Catalog # Availability Size / Price Qty
MAB1063
MAB1063-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human Coagulation Factor X Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor X in direct ELISAs and Western blots. It recognizes both Factor X and Factor Xa.
Source
Monoclonal Mouse IgG1 Clone # 156106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor X
Leu24-Lys488 (predicted)
Accession # P00742
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL

Recombinant Human Coagulation Factor X (Catalog # 1063-SE)
under non-reducing conditions only

Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor X (Catalog # 1063-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor X

As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X (rhFX) is initially synthesized in the liver as a single-chain precursor of 488 amino acids (aa) with a signal peptide and a pro region (aa 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (aa 41‑179) and heavy (aa 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain.

Entrez Gene IDs
2159 (Human); 14058 (Mouse); 29243 (Rat)
Alternate Names
Cf10; Coagulation Factor X; EC 3.4.21; EC 3.4.21.6; F10; factor Xa; FX; FXa; Prothrombinase; Stuart Factor; Stuart-Prower factor

Product Datasheets

Citation for Human Coagulation Factor X Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Protease-activated receptor 2 blocking peptide counteracts endotoxin-induced inflammation and coagulation and ameliorates renal fibrin deposition in a rat model of acute renal failure.
    Authors: Jesmin S, Gando S, Zaedi S, Prodhan SH, Sawamura A, Miyauchi T, Hiroe M, Yamaguchi N
    Shock, 2009;32(6):626-32.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Coagulation Factor X Antibody

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Human Coagulation Factor X Antibody
By Anonymous on 10/22/2019
Application: WB Sample Tested: Purified protein Species: Human