Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 156106
View all Coagulation Factor X Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor X
Leu24-Lys488 (predicted)
Accession # P00742

Specificity

Detects human Coagulation Factor X in direct ELISAs and Western blots. It recognizes both Factor X and Factor Xa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Coagulation Factor X Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor X (Catalog # 1063-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample:

Recombinant Human Coagulation Factor X (Catalog # 1063-SE)
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB1063 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor X

As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X (rhFX) is initially synthesized in the liver as a single-chain precursor of 488 amino acids (aa) with a signal peptide and a pro region (aa 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (aa 41‑179) and heavy (aa 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain.

Alternate Names

Cf10, F10, FX, FXa, Prothrombinase, Stuart Factor

Entrez Gene IDs

2159 (Human); 14058 (Mouse); 29243 (Rat)

Gene Symbol

F10

UniProt

P00742

Additional Coagulation Factor X Products

Product Documents for Human Coagulation Factor X Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Coagulation Factor X Antibody

For research use only

Citations for Human Coagulation Factor X Antibody

Customer Reviews for Human Coagulation Factor X Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human Coagulation Factor X Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 10/22/2019
    Human Coagulation Factor X Antibody MAB1063

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail