Human Coagulation Factor XI Antibody

Catalog # Availability Size / Price Qty
AF2460
AF2460-SP
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Human Coagulation Factor XI Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor XI in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse F11 is observed and less than 5% cross‑reactivity with recombinant human (rh) F7 and rhF10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor XI
Glu19-Val625
Accession # P03951
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Coagulation Factor XI (Catalog # 2460-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor XI (Catalog # 2460-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Coagulation Factor XI (2 µg/mL, Catalog # 2460-SE) cleavage of the fluorogenic peptide substrate Boc-IEGR-AMC (100 µM). The Neutralization Dose (ND50) is typically 23 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor XI

Coagulation factors XI and XIa refer to the pro and active forms of the same protease, respectively (1). Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa. Factor Xa in turn activates factor II/thrombin to complete the coagulation cascade. Patients with factor XI deficiency are prone to excessive bleeding after hemostatic challenge. There are two alternative splicing forms. Isoform 1 corresponds to the circulating plasma factor XI and isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells (2). The 625 amino acid precursor of isoform 1 consists of a signal peptide (residues 1 to 18) and the mature chain (residues 19 to 625). The mature chain (XI) can be further processed into the heavy chain (residues 19 to 387) and the light chain (residues 388 to 625) (XIa). The purified rhFactor XI corresponds to isoform 1 (residues 19 to 625), which can be activated by treatment with thermolysin.

References
  1. Wash, P.N. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1651.
  2. Hsu, T.C. et al. (1998) J. Biol. Chem. 273:13787.
Entrez Gene IDs
2160 (Human); 109821 (Mouse)
Alternate Names
Coagulation Factor XI; EC 3.4.21; EC 3.4.21.27; FXIPlasma thromboplastin antecedent; MGC141891; PTA

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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