Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, ELISA, Immunocytochemistry, Mass Spectrometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 390513
View all Complement Factor H-related 5/CFHR5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor H-related 5/CFHR5
Glu19-Glu569
Accession # Q9BXR6

Specificity

Detects human Complement Factor H-related 5/CFHR5 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Complement Factor H-related 5/CFHR5 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor H‑related 5/CFHR5 (Catalog # 3845-F5), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Complement Factor H-related 5/CFHR5 (Catalog # 3845-F5) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H-related 5/CFHR5

Complement factor H-related protein 5 (FHR-5 or CFHR5) is a 65 kDa member of the factor H-related protein family.  It is secreted by the liver and circulates either free or bound to HDL.  It is unique among FHR proteins in that it associates with complexes that arise from complement activation.  It promotes C3b cleavage and blocks C3 convertase activity.  Human CFHR5 is 551 amino acids (aa) in length.  It contains nine, 60 aa SUSHI-, or short consensus repeats, plus 18 intrachain disulfide bonds. SUSHI repeats 5-7 (aa 267-444) reportedly mediate CFHR5 binding to CRP and heparin.  The complement interaction site(s) is unknown.  Human CFHR5 shares 64% aa sequence identity with canine CFHR5.

Alternate Names

CFHL5, CFHR5, Complement Factor Hrelated 5, FHR5

Entrez Gene IDs

81494 (Human)

Gene Symbol

CFHR5

UniProt

Q9BXR6

Additional Complement Factor H-related 5/CFHR5 Products

Product Documents for Human Complement Factor H-related 5/CFHR5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Factor H-related 5/CFHR5 Antibody

For research use only

Citations for Human Complement Factor H-related 5/
CFHR5 Antibody

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