Detection of Human Copeptin by Western Blot.
Western blot shows lysates of human kidney (cortex) tissue and human kidney (medulla) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Copeptin Monoclonal Antibody (Catalog # MAB6077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Copeptin at approximately 7-10 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Copeptin is a 39 amino acid glycosylated peptide that is a proteolytic product derived from a precursor which also contains Vasopressin and Neurophysin 2 peptides. The precursor protein is synthesized and cleaved in the hypothalamus before transport to the pituitary for storage and release. Copeptin is secreted in equimolar amounts with Vasopressin. It is more stable than Vasopressin and serves as a surrogate indicator of Vasopressin release. Vasopressin plays a major role in blood pressure regulation through control of water retention in the kidney and vascular tone. Serum levels of Copeptin are associated with metabolic syndrome, insulin resistance, sepsis, and heart dysfunction following myocardial infarction. Human Copeptin shares 79% sequence identity with mouse and rat Copeptin.
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