Human CRELD1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CRELD-1 in ELISA.
  • Source
    Monoclonal Mouse IgG2B Clone # 842314
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CRELD-1
    Gln30-Glu362
    Accession # Q96HD1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize CRELD1-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.15-0.75 μg/mL in the presence of 2 μg/mL Recombinant Human CRELD1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Adhesion Mediated by CRELD1 and Neutralization by Human CRELD1 Antibody.

Recombinant Human CRELD1 (Catalog # 3128-CR), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CRELD1 (2 μg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CRELD1 Monoclonal Antibody (Catalog # MAB3128). The ND50 is typically 0.15‑0.75 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRELD1
CRELD1, also known as cirrin, is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain with two EGF-like repeats, 2 transmembrane domains separated by a very short cytoplasmic loop, and a short extracellular C-terminal domain. An alternatively spliced isoform with a unique C-terminus that lacks the transmembrane domains has also been cloned. Missense mutations in CRELD1 have been associated with atrioventricular septal defects. The N-terminal extracellular domain of human and mouse CRELD1 share 92% aa sequence identity.
  • Long Name:
    Cysteine-rich Protein With EGF-like Domains 1
  • Entrez Gene IDs:
    78987 (Human); 171508 (Mouse); 312638 (Rat)
  • Alternate Names:
    atrioventricular septal defect 2; AVSD2; CIRRIN; CRELD1; cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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