Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human CRELD-1 Gln30-Glu362 Accession # Q96HD1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CRELD1-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.15-0.75 μg/mL in the presence of 2 μg/mL Recombinant Human CRELD1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Adhesion Mediated by CRELD1 and Neutralization by Human CRELD1 Antibody.
Recombinant Human CRELD1 (Catalog # 3128-CR), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CRELD1 (2 μg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CRELD1 Monoclonal Antibody (Catalog # MAB3128). The ND50 is typically 0.15‑0.75 μg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CRELD1, also known as cirrin, is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain with two EGF-like repeats, 2 transmembrane domains separated by a very short cytoplasmic loop, and a short extracellular C-terminal domain. An alternatively spliced isoform with a unique C-terminus that lacks the transmembrane domains has also been cloned. Missense mutations in CRELD1 have been associated with atrioventricular septal defects. The N-terminal extracellular domain of human and mouse CRELD1 share 92% aa sequence identity.
Cysteine-rich Protein With EGF-like Domains 1
Entrez Gene IDs:
78987 (Human); 171508 (Mouse); 312638 (Rat)
atrioventricular septal defect 2; AVSD2; CIRRIN; CRELD1; cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213
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