CTGF/CCN2 in Human Breast Cancer Tissue. CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human CTGF/CCN2 C-Terminus Monoclonal Antibody (Catalog # MAB660) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins
that share a common multimodular organization. Each protein contains
IGF-binding protein domain, a von Willebrand factor type C domain, a
thrombospondin type I domain, and a cysteine knot domain. The
multimodular CTGF has the ability to bind multiple ligands and has
numerous effects on development, differentation, and disease. The
C-terminal cysteine knot motif contains the heparin and low density
lipoprotein receptor (LDLR) binding sites that contributes to the
adhesive and mitogenic properties of CTGF.
Connective Tissue Growth Factor
Entrez Gene IDs:
1490 (Human); 14219 (Mouse); 64032 (Rat)
CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.