Human Cyclin D1 Antibody Summary
Accession # P24385
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cyclin D1 in WM-115 and Daudi Human Cell Lines. Cyclin D1 was detected in immersion fixed WM‑115 human malignant melanoma cell line (positive stain) and Daudi human Burkitt's lymphoma cell line (negative stain) using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB43142) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin D1
Cyclin D1 (CCND1) is a 36 kDa cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.
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