Detection of Human CYP3A4 by Western Blot.
Western blot shows lysate of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CYP3A4 Monoclonal Antibody (Catalog # MAB9079) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CYP3A4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(cytochrome P450, subfamily 3A, polypeptide 4) belongs to the cytochrome P450
superfamily of monooxygenases that catalyze reactions involved in both drug
metabolism and the synthesis of cholesterol, steroids, and other lipid
components. CYP3A4 is predominantly
found in liver microsomes, but has also been detected in prostate, small and
large intestine, bile duct, nasal mucosa, kidney and adrenal cortex. It is an extremely important enzyme for drug
metabolism; CYP3A4 catalyzes phase I oxidation reactions in an estimated 60% of
all clinically used drugs, promoting in most cases their clearance by
excretion. CYP3A4 levels are induced by
glucocorticoids and various xenobiotics, including drugs, pesticides and
carcinogens. Human CYP3A4 is a 50 kDa
single-pass membrane protein, 503 amino acids in length.
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