Human CYP3A4 Antibody Summary
Accession # P08684
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CYP3A4 by Western Blot. Western blot shows lysate of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CYP3A4 Monoclonal Antibody (Catalog # MAB9079) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CYP3A4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CYP3A4 by Simple WesternTM. Simple Western lane view shows lysates of Human Liver, loaded at 0.2 mg/mL. A specific band was detected for CYP3A4 at approximately 55 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CYP3A4 Monoclonal Antibody (Catalog # MAB9079). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CYP3A4 (cytochrome P450, subfamily 3A, polypeptide 4) belongs to the cytochrome P450 superfamily of monooxygenases that catalyze reactions involved in both drug metabolism and the synthesis of cholesterol, steroids, and other lipid components. CYP3A4 is predominantly found in liver microsomes, but has also been detected in prostate, small and large intestine, bile duct, nasal mucosa, kidney and adrenal cortex. It is an extremely important enzyme for drug metabolism; CYP3A4 catalyzes phase I oxidation reactions in an estimated 60% of all clinically used drugs, promoting in most cases their clearance by excretion. CYP3A4 levels are induced by glucocorticoids and various xenobiotics, including drugs, pesticides and carcinogens. Human CYP3A4 is a 50 kDa single-pass membrane protein, 503 amino acids in length.
Citation for Human CYP3A4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary western blot technology and total protein normalization
Authors: JM Collins, D Wang
Journal of pharmacological and toxicological methods, 2021;112(0):107117.
Sample Types: Tissue Lysates
Applications: Simple Western
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