Human CYP7A1 Antibody MAB9120-100: R&D Systems

Human CYP7A1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CYP7A1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 946902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CYP7A1 synthetic peptide
    Accession # P22680
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CYP7A1 in Human Hepatocytes. CYP7A1 was detected in immersion fixed human hepatocytes induced with dexamethasone for 48 hours using Mouse Anti-Human CYP7A1 Polyclonal Antibody (Catalog # MAB9120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (green). Specific staining was localized to endoplasmic reticula and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CYP7A1
CYP7A1 (cytochrome P450 7A1; also Cholesterol 7-alph-monooxygenase) is a 504 aa oxidoreductase enzyme (SwissProt #: P22680).  Human CYP7A1 shares 81% and 82% aa identity with mouse and rat CYP7A1, respectively.  It is expressed in the liver where it catalyzes cholesterol metabolism, which is the rate-limiting step in bile acid synthesis.  Disruption of CYP7A1 is associated with high plasma cholesterol levels leading to increased risk of coronary artery disease.
  • Long Name:
    Cytochrome P450 7A1
  • Entrez Gene IDs:
    1581 (Human); 13122 (Mouse); 25428 (Rat)
  • Alternate Names:
    Cholesterol 7-alpha-hydroxylase; cholesterol 7-alpha-monooxygenase; CP7A; CYP7; CYP7A1; CYP7MGC126826; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase); cytochrome P450, subfamily VIIA polypeptide 1; EC 1.14.13; EC 1.14.13.17; MGC138389; polypeptide 1
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