Human CYP7A1 Antibody

Catalog # Availability Size / Price Qty
    CYP7A1  in Human Hepatocytes.
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Product Details
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Human CYP7A1 Antibody Summary

Species Reactivity
Detects human CYP7A1 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 946902
Protein A or G purified from hybridoma culture supernatant
Human CYP7A1 synthetic peptide
Accession # P22680
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry CYP7A1  in Human Hepatocytes. View Larger

CYP7A1 in Human Hepatocytes. CYP7A1 was detected in immersion fixed human hepatocytes induced with dexamethasone for 48 hours using Mouse Anti-Human CYP7A1 Polyclonal Antibody (Catalog # MAB9120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (green). Specific staining was localized to endoplasmic reticula and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CYP7A1

CYP7A1 (cytochrome P450 7A1; also Cholesterol 7-alph-monooxygenase) is a 504 aa oxidoreductase enzyme (SwissProt #: P22680).  Human CYP7A1 shares 81% and 82% aa identity with mouse and rat CYP7A1, respectively.  It is expressed in the liver where it catalyzes cholesterol metabolism, which is the rate-limiting step in bile acid synthesis.  Disruption of CYP7A1 is associated with high plasma cholesterol levels leading to increased risk of coronary artery disease.

Long Name
Cytochrome P450 7A1
Entrez Gene IDs
1581 (Human); 13122 (Mouse); 25428 (Rat)
Alternate Names
Cholesterol 7-alpha-hydroxylase; cholesterol 7-alpha-monooxygenase; CP7A; CYP7; CYP7A1; CYP7MGC126826; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase); cytochrome P450, subfamily VIIA polypeptide 1; EC 1.14.13; EC; MGC138389; polypeptide 1

Product Datasheets


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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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