Human CYP7A1 Antibody Summary
Accession # P22680
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CYP7A1 in Human Hepatocytes. CYP7A1 was detected in immersion fixed human hepatocytes induced with dexamethasone for 48 hours using Mouse Anti-Human CYP7A1 Polyclonal Antibody (Catalog # MAB9120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (green). Specific staining was localized to endoplasmic reticula and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CYP7A1 (cytochrome P450 7A1; also Cholesterol 7-alph-monooxygenase) is a 504 aa oxidoreductase enzyme (SwissProt #: P22680). Human CYP7A1 shares 81% and 82% aa identity with mouse and rat CYP7A1, respectively. It is expressed in the liver where it catalyzes cholesterol metabolism, which is the rate-limiting step in bile acid synthesis. Disruption of CYP7A1 is associated with high plasma cholesterol levels leading to increased risk of coronary artery disease.
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