Human Cytosolic beta-Glucosidase/GBA3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Cytosolic  beta ‑Glucosidase/GBA3 by Western Blot.
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Product Details
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Human Cytosolic beta-Glucosidase/GBA3 Antibody Summary

Species Reactivity
Detects human Cytosolic beta -Glucosidase/GBA3 in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 728714
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta -Glucosidase/GBA3
Accession # Q9H227
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cytosolic  beta -Glucosidase/GBA3 antibody by Western Blot. View Larger

Detection of Human Cytosolic beta ‑Glucosidase/GBA3 by Western Blot. Western blot shows lysates of human liver tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cytosolic beta -Glucosidase/GBA3 Monoclonal Antibody (Catalog # MAB5969) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic beta -Glucosidase/GBA3 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic beta-Glucosidase/GBA3

There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).

  1. Tybulewicz, V.L. et al. (1992) Nature 357:407.
  2. Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
  3. de Graaf, M. et al. (2001) Biochem. J. 356:907.
  4. Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.
Entrez Gene IDs
57733 (Human); 289687 (Rat)
Alternate Names
CBG; CBGL1; CBGL1GLUC; Cytosolic betaGlucosidase; Cytosolic beta-Glucosidase; Cytosolic beta-glucosidase-like protein 1; EC; GBA3; GLUC; glucosidase, beta, acid 3 (cytosolic); klotho-related protein; KLrP; MGC104276; MGC126878

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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