Human Cytosolic beta-Glucosidase/GBA3 Antibody (728714)

R&D Systems | Catalog # MAB5969

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 728714
View all Cytosolic beta-Glucosidase/GBA3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta -Glucosidase/GBA3
Thr13-Leu469
Accession # Q9H227

Specificity

Detects human Cytosolic beta -Glucosidase/GBA3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cytosolic beta-Glucosidase/GBA3 Antibody (728714)

Detection of Human Cytosolic beta -Glucosidase/GBA3 antibody by Western Blot.

Detection of Human Cytosolic beta ‑Glucosidase/GBA3 by Western Blot.

Western blot shows lysates of human liver tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cytosolic beta -Glucosidase/GBA3 Monoclonal Antibody (Catalog # MAB5969) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic beta -Glucosidase/GBA3 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Cytosolic beta-Glucosidase/GBA3 Antibody (728714)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human liver tissue and human kidney tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic beta-Glucosidase/GBA3

There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).

References

  1. Tybulewicz, V.L. et al. (1992) Nature 357:407.
  2. Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
  3. de Graaf, M. et al. (2001) Biochem. J. 356:907.
  4. Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.

Alternate Names

CBGL1, Cytosolic betaGlucosidase, GBA3, GLUC, KLrP

Entrez Gene IDs

57733 (Human); 289687 (Rat)

Gene Symbol

GBA3

UniProt

Q9H227 (Human)

Additional Cytosolic beta-Glucosidase/GBA3 Products

Product Documents for Human Cytosolic beta-Glucosidase/GBA3 Antibody (728714)

Certificate of Analysis

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Product Specific Notices for Human Cytosolic beta-Glucosidase/GBA3 Antibody (728714)

For research use only

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