Human DAPK3/ZIPK Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human DAPK3 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DAPK3
    Arg347-Arg454
    Accession # O43293
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human DAPK3/ZIPK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DAPK3/ZIPK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DAPK3/ZIPK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DAPK3/ZIPK

DAPK3 (Death-associated protein kinase 3; also known as ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes. In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic. DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation. Human DAPK3 is 454 amino acids (aa) in length and contains one protein kinase domain (aa 134‑275) and a C-terminal Leu-zipper motif (aa 427‑441) that mediates homo- and hetero-dimerization. There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313‑454. This form has both a nuclear and cytosolic localization.

  • Long Name:
    Death-associated Protein Kinase 3
  • Entrez Gene IDs:
    1613 (Human); 13144 (Mouse); 64391 (Rat)
  • Alternate Names:
    DAP kinase 3; DAPK3; DAP-like kinase; death-associated protein kinase 3; Dlk; EC 2.7.11; EC 2.7.11.1; ZIP kinase isoform; ZIP; ZIPK; ZIPKFLJ36473; ZIP-kinase
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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