Human DAPK3/ZIPK Antibody

Catalog # Availability Size / Price Qty
Detection of Human DAPK3/ZIPK by Western Blot.
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Product Details
Supplemental Products

Human DAPK3/ZIPK Antibody Summary

Species Reactivity
Detects endogenous human DAPK3 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human DAPK3
Accession # O43293
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DAPK3/ZIPK antibody by Western Blot. View Larger

Detection of Human DAPK3/ZIPK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DAPK3/ZIPK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DAPK3/ZIPK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAPK3/ZIPK

DAPK3 (Death-associated protein kinase 3; also known as ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes. In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic. DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation. Human DAPK3 is 454 amino acids (aa) in length and contains one protein kinase domain (aa 134‑275) and a C-terminal Leu-zipper motif (aa 427‑441) that mediates homo- and hetero-dimerization. There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313‑454. This form has both a nuclear and cytosolic localization.

Long Name
Death-associated Protein Kinase 3
Entrez Gene IDs
1613 (Human); 13144 (Mouse); 64391 (Rat)
Alternate Names
DAP kinase 3; DAPK3; DAP-like kinase; death-associated protein kinase 3; Dlk; EC 2.7.11; EC; ZIP kinase isoform; ZIP; ZIPK; ZIPKFLJ36473; ZIP-kinase

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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