Human DGAT2 Antibody Summary
Accession # Q96PD7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
DGAT2 in HepG2 Human Cell Line. DGAT2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human DGAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7256) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DGAT2 in Human Liver. DGAT2 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human DGAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7256) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DGAT2 (Diacylglycerol O-acyltransferase 2; also DGAT2 alpha ) is a 45-50 kDa, insulin-regulated member of the DGAT2 family of enzymes. Although DGAT1 also exists, it is related to DGAT2 only by function; DGAT1 and DGAT2 are members of structurally unrelated gene families. DGAT2 catalyzes the formation of triglyceride (TG) from sn-1, 2-DG and a variety of acyl-CoAs. It is found in sebaceous gland cells, adipocytes, and hepatocytes, and appears to synthesize TGs for incorporation into VLDL particles. Human DGAT2 is a 388 amino acid (aa) multipass ER protein. It contains a 69 aa N-terminal cytoplasmic region, a short luminal sequence (aa 89-92) and a long C-terminal cytoplasmic domain that contains acyl transferase activity (aa 113-388). DGAT2 does homomultimerize, forming 680 kDa complexes. There is one splice variant that contains a deletion of aa 41-83. Over aa 268-377, human DGAT2 shares 95% aa sequence identity with mouse DGAT2.
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Cell and Tissue Staining Kits
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