Human DGAT2 Antibody

Catalog # Availability Size / Price Qty
AF7256
AF7256-SP
DGAT2 in HepG2 Human Cell Line.
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Human DGAT2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DGAT2 in direct ELISAs. In direct ELISAs, approximately 7% cross-reactivity with recombinant human DGAT2-L6 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DGAT2
Arg268-Phe377
Accession # Q96PD7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry DGAT2 in HepG2 Human Cell Line. View Larger

DGAT2 in HepG2 Human Cell Line. DGAT2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human DGAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7256) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry DGAT2 in Human Liver. View Larger

DGAT2 in Human Liver. DGAT2 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human DGAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7256) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGAT2

DGAT2 (Diacylglycerol O-acyltransferase 2; also DGAT2 alpha ) is a 45-50 kDa, insulin-regulated member of the DGAT2 family of enzymes. Although DGAT1 also exists, it is related to DGAT2 only by function; DGAT1 and DGAT2 are members of structurally unrelated gene families. DGAT2 catalyzes the formation of triglyceride (TG) from sn-1, 2-DG and a variety of acyl-CoAs. It is found in sebaceous gland cells, adipocytes, and hepatocytes, and appears to synthesize TGs for incorporation into VLDL particles. Human DGAT2 is a 388 amino acid (aa) multipass ER protein. It contains a 69 aa N-terminal cytoplasmic region, a short luminal sequence (aa 89-92) and a long C-terminal cytoplasmic domain that contains acyl transferase activity (aa 113-388). DGAT2 does homomultimerize, forming 680 kDa complexes. There is one splice variant that contains a deletion of aa 41-83. Over aa 268-377, human DGAT2 shares 95% aa sequence identity with mouse DGAT2.

Long Name
Diacylglycerol O-Acyltransferase 2
Entrez Gene IDs
84649 (Human); 67800 (Mouse); 252900 (Rat)
Alternate Names
DGAT2; diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase homolog 2 (mouse); diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; Diglyceride acyltransferase 2; DKFZp686A15125; EC 2.3.1; EC 2.3.1.20; GS1999FULL

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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