Human Dkk-1 Antibody (141119)

R&D Systems | Catalog # MAB1096

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 141119
View all Dkk-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907

Specificity

Detects human Dkk-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) Dkk-1 and no cross-reactivity with rmDkk-2, rhDkk-3, or rhDkk-4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Dkk-1 Antibody (141119)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Dkk‑1 (Catalog # 5439-DK)
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB1096 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-1

Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

UniProt

O94907

Additional Dkk-1 Products

Product Documents for Human Dkk-1 Antibody (141119)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Dkk-1 Antibody (141119)

For research use only

Citations for Human Dkk-1 Antibody (141119)

Customer Reviews for Human Dkk-1 Antibody (141119) (1)

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  • Human Dkk-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: DKK-1 overexpressed HEK293 cells
    Species: Human
    Verified Customer | Posted 08/25/2021
    1:1000 dilution.
    Human Dkk-1 Antibody (141119) MAB1096

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