Human Dopamine D5 R/DRD5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects HEK293 cells transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Mouse IgG1 Clone # 889040
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
    Accession # P21918
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

Dopamine D5 R/DRD5 in Human Brain. Dopamine D5 R/DRD5 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB8286) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopamine D5 R/DRD5
DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of protein kinase C (PKC) (6).
  • References:
    1. Sunahra, R. et al. (1991) Nature.  350:614.
    2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
    3. Squassina, A. et al.  (2008) Neurosci Lett.  Feb 13.
    4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
    5. Wei, J. et al.  (2012) Addict Behav. 37:622.
    6. Thompson, D. et al. (2011) Traffic. 12:644.
  • Long Name:
    Dopamine D5 Receptor
  • Entrez Gene IDs:
    1816 (Human); 13492 (Mouse); 25195 (Rat)
  • Alternate Names:
    D(1B) dopamine receptor; D(5) dopamine receptor; D1beta dopamine receptor; DBDR; Dopamine D5 R; Dopamine D5 receptor; Dopamine D5R; dopamine receptor D1B; dopamine receptor D5; DRD1B; DRD1BDBDR; DRD1L2; DRD1L2MGC10601; DRD5
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