Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 889040
View all Dopamine D5R/DRD5 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
Accession # P21918

Specificity

Detects HEK293 cells transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Dopamine D5R/DRD5 Antibody

Dopamine D5 R/DRD5 antibody in Human Brain by Immunohistochemistry (IHC-P).

Dopamine D5 R/DRD5 in Human Brain.

Dopamine D5 R/DRD5 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB8286) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Dopamine D5R/DRD5 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dopamine D5R/DRD5

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of protein kinase C (PKC) (6).

References

  1. Sunahra, R. et al. (1991) Nature.  350:614.
  2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
  3. Squassina, A. et al.  (2008) Neurosci Lett.  Feb 13.
  4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
  5. Wei, J. et al.  (2012) Addict Behav. 37:622.
  6. Thompson, D. et al. (2011) Traffic. 12:644.

Long Name

Dopamine D5 Receptor

Alternate Names

DBDR, Dopamine D5 R, DRD1B, DRD1L2, DRD5

Entrez Gene IDs

1816 (Human); 13492 (Mouse); 25195 (Rat)

Gene Symbol

DRD5

UniProt

P21918

Additional Dopamine D5R/DRD5 Products

Product Documents for Human Dopamine D5R/DRD5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Dopamine D5R/DRD5 Antibody

For research use only

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Protocols

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