Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DRAK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DRAK1
Ala115-Glu313
Accession # Q9UEE5

Specificity

Detects human DRAK1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human DRAK2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DRAK1 Antibody

Detection of Human DRAK1 antibody by Western Blot.

Detection of Human DRAK1 by Western Blot.

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DRAK1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DRAK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: JEG‑3 human epithelial choriocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DRAK1

DRAK1 (DAP kinase-related apoptosis-inducing protein kinase 1; also STK17A) is a 55 kDa member of the DAP kinase subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in osteoclasts, resides in the nucleus and initiates apoptosis. Human DRAK1 is 414 amino acids (aa) in length. It contains an N‑terminal polyPro segment (aa 33‑37) followed by a Ser/Thr kinase domain (aa 64‑321) with a catalytic loop (aa 184‑193). Apoptosis apparently requires an intact kinase domain. No rodent DRAK1 has been reported. Over aa 115‑313, human DRAK1 is 95% aa identical to canine DRAK1, and 65% aa identical to human DRAK2.

Long Name

DAP Kinase-related Apoptosis-inducing Protein Kinase 1

Alternate Names

STK17A

Entrez Gene IDs

9263 (Human)

Gene Symbol

STK17A

UniProt

Q9UEE5

Additional DRAK1 Products

Product Documents for Human DRAK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DRAK1 Antibody

For research use only

Related Research Areas

Apoptosis Intracellular Kinases

Customer Reviews for Human DRAK1 Antibody

There are currently no reviews for this product. Be the first to review Human DRAK1 Antibody and earn rewards!

Have you used Human DRAK1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies