DSCAM-L1 is a 250 kDa type I transmembrane Ig superfamily glycoprotein that is predominantly expressed in Purkinje cells and neurons of the cerebral cortex and olfactory bulb.DSCAM-L1 mediates cell adhesion by cation-independent homophilic interactions. It may function in the formation and maintenance of neural networks and play a role in neural disorders such as Gilles de la Tourette and Jacobsen syndromes. Human DSCAM-L1 contains ten Ig-like C2-type domains and six fibronectin type-III domains. An alternately spliced isoform is generated by deletion of 211 amino acids near the N-terminal. Within the extracellular domain, human DSCAM-L1 shares 98% amino acid sequence identity with mouse and rat DSCAM-L1.
Human DSCAM‑L1 Antibody
R&D Systems | Catalog # AF3315
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DSCAM-L1
Glu19-Lys1591
Accession # Q8TD84
Glu19-Lys1591
Accession # Q8TD84
Specificity
Detects human DSCAM-L1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human DSCAM is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DSCAM‑L1 Antibody
Detection of DSCAM‑L1 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Goat Anti-Human DSCAM-L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3315, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Applications for Human DSCAM‑L1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: A172 human glioblastoma cell line
Sample: A172 human glioblastoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human DSCAM-L1
Sample: Recombinant Human DSCAM-L1
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DSCAM-L1
Long Name
Down Syndrome Cell Adhesion Molecule Like 1
Alternate Names
DSCAML1
Gene Symbol
DSCAML1
UniProt
Additional DSCAM-L1 Products
Product Documents for Human DSCAM‑L1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human DSCAM‑L1 Antibody
For research use only
Related Research Areas
Citations for Human DSCAM‑L1 Antibody
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