Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all E2F-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human E2F-4
Lys178-Asp306
Accession # Q16254

Specificity

Detects endogenous human E2F-4 in Western bots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human E2F‑4 Antibody

Detection of Human E2F‑4 antibody by Western Blot.

Detection of Human E2F‑4 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human E2F-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5139) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for E2F-4 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human E2F‑4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E2F-4

E2F-4 (viral E2-associated factor 4) is a 50 kDa member of the E2F/DP family of transcription factors. It is ubiquitously and constitutively expressed, and forms a DNA-binding E2F heterodimeric complex with DP-1. This complex, when associated with p130/RBL2 and a series of LIN-numbered proteins, represses cell cycle‑dependent genes during G0 and G1. Human E2F-4 is 413 amino acids (aa) in length and contains a poly-Pro region (aa 9‑12), a DNA-binding domain (aa 16‑85), a dimerization segment (aa 86‑181), poly-Ser region (aa 307‑327), and an RBL1/RBL2 binding domain (aa 390‑407). There is one potential alternate start site at Met261. Over aa 178‑306, human E2F-4 shares 80% and 83% aa identity with mouse and canine E2F-4, respectively.

Long Name

E2F Transcription Factor 4

Alternate Names

E2F4

Entrez Gene IDs

1874 (Human); 104394 (Mouse)

Gene Symbol

E2F4

UniProt

Q16254

Additional E2F-4 Products

Product Documents for Human E2F‑4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human E2F‑4 Antibody

For research use only

Citations for Human E2F‑4 Antibody

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