Detection of Human E2F‑4 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human E2F‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5139) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for E2F‑4 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
E2F-4 (viral E2-associated factor 4) is a 50 kDa member of the E2F/DP family of transcription factors. It is ubiquitously and constitutively expressed, and forms a DNA-binding E2F heterodimeric complex with DP-1. This complex, when associated with p130/RBL2 and a series of LIN-numbered proteins, represses cell cycle‑dependent genes during G0 and G1. Human E2F-4 is 413 amino acids (aa) in length and contains a poly-Pro region (aa 9‑12), a DNA-binding domain (aa 16‑85), a dimerization segment (aa 86‑181), poly-Ser region (aa 307‑327), and an RBL1/RBL2 binding domain (aa 390‑407). There is one potential alternate start site at Met261. Over aa 178‑306, human E2F-4 shares 80% and 83% aa identity with mouse and canine E2F-4, respectively.
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