Human ErbB2/Her2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1129
ErbB2/Her2 in MCF-7 Human Cell Line.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human ErbB2/Her2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ErbB2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) ErbB3, rhErbB4, and rhEGF R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB2
Thr23-Thr652
Accession # P04626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Human ErbB2/Her2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human ErbB2/Her2 Antibody (Catalog # MAB1129)

Standard: Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF (Catalog # 1129-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry ErbB2/Her2 in MCF-7 Human Cell Line. View Larger

ErbB2/Her2 in MCF-7 Human Cell Line. ErbB2/Her2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ErbB2/Her2 in Human Breast Cancer Tissue. View Larger

ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

References
  1. Coussens, L. et al. (1985) Science 230:1132.
  2. Yamamoto, T. et al. (1986) Nature 319:230.
  3. Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Product Datasheets

Citations for Human ErbB2/Her2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Molecular nanoshearing: an innovative approach to shear off molecules with AC-induced nanoscopic fluid flow.
    Authors: Shiddiky, Muhammad, Vaidyanathan, Ramanath, Rauf, Sakandar, Tay, Zhikai, Trau, Matt
    Sci Rep, 2014;4(0):3716.
    Species: Human
    Sample Types: Serum
    Applications: Functional Assay
  2. Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
    Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
    Assay Drug Dev Technol, 2010;8(1):27-36.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Luminex Development
  3. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development

FAQs

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Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Staining Reagents

Avidin-Fluorescein

Supplemental Cell Selection Products

Supplemental ELISA Products

Reviews for Human ErbB2/Her2 Biotinylated Antibody

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Human ErbB2/Her2 Biotinylated Antibody
By Anonymous on 02/03/2016
Application: Immunocytochemistry/Immunoflourescence Sample Tested: SK-BR-3 human breast cancer cell line Species: Goat

SK-BR-3 cell staining with Biotin-labeled pAb (BAF1129) and Streptavidin, Alexa Fluor® 633 conjugate.