EZFIT (Endothelial Zinc Finger Protein Induced by TNF-alpha) is a member of the Kruppel C2H2-type zinc finger protein family of DNA binding molecules. It is an intracellular protein that contains thirteen Cys/His-based zinc fingers. Each zinc finger is 22 amino acids in size and binds one zinc ion. Zinc finger motifs are interconnected by highly conserved peptide motifs called Kruppel-links. Each Kruppel-link helps align zinc finger domains in the major groove of a DNA helix.
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Direct ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # K9716
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Product Specifications
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human EZFIT aa 6-199 Antibody
Application
Recommended Usage
Direct ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: EZFIT
Long Name
Endothelial Zinc Finger Protein Induced by TNF-alpha
Alternate Names
Cos26, ZNF47, ZNF71
Entrez Gene IDs
58491 (Human)
Gene Symbol
ZNF71
Additional EZFIT Products
Product Documents for Human EZFIT aa 6-199 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human EZFIT aa 6-199 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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