Human FAM20B Antibody

Catalog # Availability Size / Price Qty
Detection of Human FAM20B by Western Blot.
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Product Details
Citations (1)
Supplemental Products
Reviews (1)

Human FAM20B Antibody Summary

Species Reactivity
Detects human FAM20B in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 1018512
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human FAM20B
Accession # O75063
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FAM20B antibody by Western Blot. View Larger

Detection of Human FAM20B by Western Blot. Western blot shows lysates of ZR-75 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAM20B Monoclonal Antibody (Catalog # MAB8427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FAM20B at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM20B

FAM20B is a member of the FAM20 protein family that has three members in mammals (FAM20A, FAM20B, FAM20C) (1). Human FAM20B shares 97% amino acid sequence identity with mouse and rat FAM20B. FAM20B localizes to the Golgi apparatus where it phosphorylates the xylose residue in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans, which leads to enhanced GAG biosynthesis (2). Accordingly, chondroitin sulfate and heparan sulfate production is increased when FAM20B is over-expressed and reduced following FAM20B knockdown (2). FAM20B knockout mice display embryonic lethality at day E13.5, suggesting that FAM20B has an important role during development (3). Furthermore, in zebrafish, FAM20B mutations result in reduced cartilage matrix production and skeletal defects (4). The enzymatic activity of recombinant human FAM20B is measured using a phosphatase-coupled method (5).

  1. Nalbant, D. et al. (2005) BMC Genomics 6:11.
  2. Koike, T. et al. (2009) Biochem. J. 421:157.
  3. Vogel, P. et al. (2012) Vet. Pathol. 49:998.
  4. Eames, B.F. et al. (2011) PLoS Genet. 7:e1002246.
  5. Wu, Z.L. (2011) PLoS One 6:e23172.
Long Name
Family with Sequence Similarity 20, Member B
Entrez Gene IDs
9917 (Human); 215015 (Mouse); 304885 (Rat)
Alternate Names
EC 2.7.1.-; FAM20B; family with sequence similarity 20, member B; Glycosaminoglycan Xylosylkinase; gxk1; KIAA0475; Protein FAM20B; XYLK; Xylose Kinase

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Citation for Human FAM20B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Altered sulfation status of FAM20C-dependent chondroitin sulfate is associated with osteosclerotic bone dysplasia
    Authors: T Koike, T Mikami, JI Tamura, H Kitagawa
    Nature Communications, 2022;13(1):7952.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot


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Human FAM20B Antibody
By Anonymous on 01/29/2022
Application: WB Sample Tested: ZR-75 human breast cancer cell line Species: Human