Human Fetuin B Antibody Summary
Accession # Q9UGM5
Human Fetuin B Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Fetuin B by Western Blot. Western blot shows human serum. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Fetuin B by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 62 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fetuin B
Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).
- Oliver, E., et al. (1999) Genomics. 57:352.
- Oliver, E., et al. (2000) Biochem. J. 350:589.
- Kellemann, J. et al., 1989, J. Biol. Chem. 264:14121.
- Denecke, B. et al. (2003) Biochem. J. 376:135.
- Schäfer, C., et al. (2003) J. Clin. Invest. 112:357.
- Dziegielewska, K. M., et al. (1996) Histochem. Cell Biol. 106:319.
- Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.
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