Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3117
View all FGF acidic/FGF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF acidic/FGF1

Specificity

Detects rhFGF acidic/FGF1 in direct ELISAs and Western blots. Does not cross-react with any other family members tested.11rhFGF3, rhFGF4, rhFGF5, rhFGF6, rhFGF7, rhFGF8b, rmFGF8b, rmFGF8c, rhFGF9, rhFGF10, rmFGF15, rhFGF17, rhFGF18, rhFGF19, bFGF-acidic, bFGF basic, rhFGF basic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human FGF acidic/FGF1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human FGF acidic aa 16-155 (Catalog # 232-FA)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist.

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Entrez Gene IDs

2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)

Gene Symbol

FGF1

Additional FGF acidic/FGF1 Products

Product Documents for Human FGF acidic/FGF1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FGF acidic/FGF1 Antibody

For research use only

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