Human FGF acidic/FGF1 Antibody

Catalog # Availability Size / Price Qty
MAB232
MAB232-SP
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Human FGF acidic/FGF1 Antibody Summary

Species Reactivity
Human
Specificity
Detects rhFGF acidic/FGF1 in direct ELISAs and Western blots. Does not cross-react with any other family members tested.1

1rhFGF3, rhFGF4, rhFGF5, rhFGF6, rhFGF7, rhFGF8b, rmFGF8b, rmFGF8c, rhFGF9, rhFGF10, rmFGF15, rhFGF17, rhFGF18, rhFGF19, bFGF-acidic, bFGF basic, rhFGF basic
Source
Monoclonal Mouse IgG1 Clone # 3117
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human FGF acidic/FGF1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF acidic aa 16-155 (Catalog # 232-FA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist.

Long Name
Fibroblast Growth Factor acidic
Entrez Gene IDs
2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
Alternate Names
AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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