Detects alpha isoforms (3 Ig-like domains) and beta isoforms (2 Ig-like domains) of human FGF R2 in direct ELISAs and Western blots. In direct ELISAs, this antibody reacts with all isoforms of rhFGF R2 but shows approximately a 4-fold preference for (IIIc) isoforms. Approximately 100% cross-reactivity with recombinant mouse FGF R2, 15% cross-reactivity with recombinant human (rh) FGF RI ( beta isoforms), and no cross-reactivity with rhFGF RI ( alpha isoforms), rhFGF R3, or rhFGF R4 is observed.
Monoclonal Mouse IgG1 Clone # 98725
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (Catalog # 663-FR) Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (Catalog # 712-FR) Recombinant Human FGF R2 beta (IIIb) Fc Chimera (Catalog # 665-FR) Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR)
2.5 µg/106 cells
Kato III human cell line
Measured by its ability to neutralize FGF R2 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 4 ng/mL Recombinant Human FGF R2 alpha (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (Catalog # 712-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (4 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB684). The ND50 is typically 1-5 µg/mL in the presence of heparin (10 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF R2
FGF R2 is a transmembrane tyrosine kinase that functions as a receptor for several FGF family proteins. The extracellular domain includes three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. Alternative exon usage results in IIIb or IIIc isoforms that differ in their membrane proximal Ig-like domain.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.