Detects human FGF R3 (IIIb) in direct ELISAs. In direct ELISAs, approximately 25‑50% cross-reactivity with recombinant mouse FGF R3 (IIIb) is observed and 12% with recombinant human (rh) FGF R4 is observed and no cross-reactivity with (IIIc) isoforms of rhFGF R3 or any isoforms of rhFGF R1 or rhFGF R2 is observed.
Monoclonal Mouse IgG1 Clone # 136315
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIb and IIIc)
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 300 ng/mL Recombinant Human FGF R3 (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R3 Antibody. Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232‑FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND50 is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF R3
Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfateproteoglycans as cofactors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9,and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.
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